📖 ABSTRACT/OVERVIEW
Type 2 diabetes mellitus is associated with impaired fracture healing characterised by delayed callus formation, reduced bone strength, and elevated non-union rates, yet the proteomic landscape of diabetic fracture callus remains uncharacterised in African patient populations. This study employs quantitative mass spectrometry-based proteomics to characterise the callus protein expression profile in type 2 diabetic patients with delayed fracture union compared to matched normoglycaemic controls at Aminu Kano Teaching Hospital and National Orthopaedic Hospital Dala, North West Nigeria. Callus biopsy samples will be obtained at six weeks post-fixation in 60 diabetic patients (40 with delayed union, 20 with normal union) and 40 matched non-diabetic controls. Protein extraction, tryptic digestion, and label-free quantitative LC-MS/MS analysis will identify differentially expressed proteins with fold changes exceeding 1.5 at a false discovery rate threshold of 5 percent. Bioinformatics analysis including STRING network analysis, gene ontology annotation, and pathway enrichment will identify disrupted healing pathways. Candidate biomarkers will be validated by ELISA in a larger serum cohort. The study hypothesises that delayed union in diabetic patients is characterised by under-expression of collagen cross-linking enzymes, impaired angiogenic signalling, and dysregulation of matrix metalloproteinase balance. This research generates an original proteomic framework for diabetic impaired fracture healing with biomarker candidates applicable to diagnostic test development and novel therapeutic targeting. Keywords: proteomics, diabetic fracture healing, delayed union, mass spectrometry, biomarkers.
Need Complete Chapters of the Above Topic?
Get high-quality, Zero-AI research materials with current citations.
Request via WhatsApp 💬